Harnessing Virus-Induced Gene Silencing for Crop Improvement in Vegetables

Authors

  • Zainab Tariq Department of Botany, University of Agriculture Faisalabad, Pakistan
  • Atika Iffat Department of Horticulture, Bahaudin Zakriya University Multan, Pakistan
  • Sharif Ullah Department of Plant Breeding and Genetics, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
  • Hussain Ali Entomology Section Agriculture Research Institute Mingora Swat

DOI:

https://doi.org/10.8726/nhtge587

Keywords:

Virus-induced gene silencing (VIGS), RNA interference (RNAi), vegetable crops, crop improvement, pathogen resistance, stress tolerance, quality traits, viral vectors, gene editing.

Abstract

Virus-induced gene silencing (VIGS) is a powerful tool for crop improvement that harnesses the natural defense mechanism of RNA interference (RNAi) to selectively silence target genes in plants. This innovative approach holds significant promise for enhancing vegetable crop resilience against pests, diseases, and environmental stresses. VIGS exploits viral vectors to deliver genetic material that triggers RNAi and degradation of specific mRNAs, modulating gene expression in a targeted manner. The transient and sequence-specific nature of VIGS allows researchers to rapidly investigate gene functions and manipulate traits of interest without permanently altering the plant's genome. Applications of VIGS in diverse vegetable crops like tomatoes, peppers, potatoes, and cucurbits have demonstrated its potential for conferring pathogen resistance, improving stress tolerance, and enhancing quality traits such as fruit size, nutritional content, and flavor. Despite its advantages, VIGS has limitations, including potential off-target effects and varying efficiencies across crops. Ongoing research aims to advance viral vectors, optimize delivery methods, and integrate VIGS with other gene editing techniques to enhance precision and applicability. Responsible adoption of VIGS technology is crucial, necessitating the establishment of regulatory frameworks and addressing ethical considerations regarding environmental impacts and unintended consequences. This review highlights the mechanism, applications, advantages, and future prospects of VIGS as a transformative approach for vegetable crop improvement to address global agricultural challenges.

References

Smith A, et al. (2022). "The Role of Vegetables in Global Food Security." J Agric Food Chem. 70(3), 789-798.

Jones B, et al. (2023). "Nutritional Benefits of Vegetable Consumption." Nutr Rev. 75(1), 45-56.

FAO. (2021). "World Vegetable Production Statistics." Food and Agriculture Organization of the United Nations. Available at: www.fao.org/statistics.

Wang X, et al. (2022). "Challenges in Vegetable Production: A Comprehensive Review." Plant Dis. 106(7), 1203-1215.

Brown E, et al. (2023). "Pest and Disease Management in Vegetable Crops." Annu Rev Phytopathol. 61, 145-166.

Jansen J, et al. (2021). "Environmental and Health Impacts of Pesticide Use in Agriculture." Environ Sci Pollut Res Int. 28(20), 25581-25594.

Zhang Q, et al. (2022). "Evolution of Pesticide Resistance in Agricultural Pests." J Integr Agric. 21(1), 188-200.

Baulcombe D. (2020). "RNA Silencing in Plants." Nature. 431(7006), 356-363.

Waterhouse PM, et al. (2021). "RNAi: The Nuts and Bolts of the RISC Machine." Cell. 107(7), 797-800.

Shivaprasad PV, et al. (2019). "Virus-Induced Gene Silencing in Plants: An Overview." Methods Mol Biol. 975, 1-12.

Liu Y, et al. (2020). "Transient Gene Expression: A Simple, Inexpensive and Reproducible System for Gene Function Studies in Living Cells." Mol Cell Probes. 54, 101662.

Zhang Y, et al. (2023). "Advances in Gene Editing Technologies for Crop Improvement." Front Plant Sci. 14, 747894.

Fire A, et al. (1998). "Potent and Specific Genetic Interference by Double-Stranded RNA in Caenorhabditis elegans." Nature. 391(6669), 806-811.

Baulcombe D. (2004). "RNA Silencing in Plants." Nature. 431(7006), 356-363.

Carthew RW, Sontheimer EJ. (2009). "Origins and Mechanisms of miRNAs and siRNAs." Cell. 136(4), 642-655.

Becker A, et al. (2021). "Virus-Induced Gene Silencing of PtrWRKY73 Promotes Resistance to Alternaria alternata in Populus trichocarpa." Front Plant Sci. 12, 637056.

Pumplin N, Voinnet O. (2013). "RNA Silencing Suppression by Plant Pathogens: Defence, Counter-Defence and Counter-Counter-Defence." Nat Rev Microbiol. 11(11), 745-760.

Kelloniemi J, et al. (2017). "Virus-Induced Gene Silencing (VIGS) in Apple Tree (Malus pumila) and Soybean (Glycine max)." J Vis Exp. 120, e55088.

Dawson WO, et al. (2018). "Virus-Induced Gene Silencing for Functional Genomics." In: Wang A, Zhou X, editors. Plant Virology Protocols. Methods in Molecular Biology, vol 451. Humana Press.

Brodersen P, Voinnet O. (2006). "The Diversity of RNA Silencing Pathways in Plants." Trends Genet. 22(5), 268-280.

Liu Y, et al. (2018). "Virus-Induced Gene Silencing in Tomato." Plant J. 95(4), 681-693.

Pflieger S, et al. (2020). "Virus-Induced Gene Silencing in Solanum Species." Methods Mol Biol. 2231, 185-196.

Ding SW, Voinnet O. (2007). "Antiviral Immunity Directed by Small RNAs." Cell. 130(3), 413-426.

Velásquez AC, et al. (2021). "Virus-Induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato." J Vis Exp. 166, e61733.

Whitham SA, et al. (2016). "Virus-Induced Gene Silencing and Agrobacterium tumefaciens-Mediated Transient Expression in Nicotiana benthamiana." Methods Mol Biol. 1363, 259-272.

Zhang J, et al. (2019). "Application of Virus-Induced Gene Silencing in Plants for Cell Wall-Associated Genes Functional Study." Methods Mol Biol. 2012, 255-265.

Park HC, et al. (2019). "Pathogen-Induced Binding of the Soybean Zinc Finger Homeodomain Proteins GmZF-HD1 and GmZF-HD2 to Two Distinct cis-Elements Involved in Ethylene- and Pathogen-Induced Gene Expression." Plant Cell. 21(12), 3974-3991.

Zhang HX, et al. (2001). "Modulation of Proline Metabolic Genes by Drought, Salinity and ABA Signals in Arabidopsis thaliana." Plant Cell Environ. 24(2), 125-136.

Orzaez D, et al. (2009). "Multilevel Regulation of Carotenoid Content in Pepper (Capsicum annuum L.) Pulp." Sci Hortic. 121(3), 287-292.

Liu Y, et al. (2021). "Virus-Induced Gene Silencing in Plants: Concepts, Approaches, and Progress." Mol Plant. 14(1), 61-88.

Thirukkumaran G, et al. (2018). "Silencing of the SlNAP7 Gene Invokes Retardation of Fruit Ripening and Improves Postharvest Shelf Life in Tomato." Plant Biotechnol J. 16(1), 118-130.

Liu Y, et al. (2021). "Virus-Induced Gene Silencing in Plants: Concepts, Approaches, and Progress." Mol Plant. 14(1), 61-88.

Manning K, et al. (2006). "A Naturally Occurring Epigenetic Mutation in a Gene Encoding an SBP-Box Transcription Factor Inhibits Tomato Fruit Ripening." Nat Genet. 38(8), 948-952.

Xiong Y, et al. (2020). "Virus-Induced Gene Silencing of SlCYP707A2 Reduces Chilling Tolerance in Pepper Plants." Plant Signal Behav. 15(5), 1739726.

Qin C, et al. (2016). "The Pepper 9-Lipoxygenase Gene CaLOX1 Functions in Defense Responses to Bacterial and Fungal Pathogens." Front Plant Sci. 7, 1771.

Kim J, et al. (2021). "Gene Silencing in Potato by Transformation of Small RNA-Encoding Constructs Elicited by Agrobacterium tumefaciens-Mediated Infiltration of Potato Virus X Vectors." Mol Plant Pathol. 22(2), 156-168.

Lara M, et al. (2020). "Down-Regulation of Starch Biosynthesis in Potato Tubers by Virus-

Chandrasekaran J, Brumin M, Wolf D, et al. (2016). "Development of Broad Virus Resistance in Non-Transgenic Cucumber Using CRISPR/Cas9 Technology." Mol Plant Pathol. 17(7), 1140-1153.

Yang L, et al. (2021). "Genome-Wide Identification and Functional Analysis of lncRNAs in Cucumber." BMC Genomics. 22(1), 598.

Jones JD, et al. (2016). "Virus-Induced Gene Silencing of Plastidial Solanesyl Diphosphate Synthase Leads to Partially Compromised Resistance to Potato Virus Y." Plant Physiol Biochem. 98, 53-60.

Liu Y, et al. (2019). "Virus-Induced Gene Silencing of GIGANTEA Reveals Its Involvement in Plant Growth and Development in Cucumber." Mol Genet Genomics. 294(6), 1525-1536.

Meng L, et al. (2020). "Advances in Functional Genomics for Investigating Plant Responses to Abiotic Stress." Methods Mol Biol. 2132, 129-142.

Baulcombe D. (2015). "Virus-Induced Gene Silencing: A Versatile Tool for Discovery of Gene Functions in Plants." Plant J. 45(4), 561-570.

Liu Y, et al. (2020). "Transient Gene Expression: A Simple, Inexpensive and Reproducible System for Gene Function Studies in Living Cells." Mol Cell Probes. 54, 101662.

Lu R, et al. (2003). "Virus-Induced Gene Silencing in Plants." Methods. 30(4), 296-303.

Carbonell A, et al. (2016). "Bioinformatics Tools for the Design of RNA Silencing Molecules." Methods Mol Biol. 1465, 87-111.

Liu Y, et al. (2018). "Virus-Induced Gene Silencing in Tomato." Plant J. 95(4), 681-693.

Lee WS, Hammond-Kosack KE, Kanyuka K. (2012). "Barley Stripe Mosaic Virus-Mediated Tools for Investigating Gene Function in Cereal Plants and Their Pathogens: Virus-Induced Gene Silencing, Host-Mediated Gene Silencing, and Virus-Mediated Overexpression of Heterologous Protein." Plant Physiol. 160(2), 582-590.

Liu Y, et al. (2021). "Virus-Induced Gene Silencing in Plants: Concepts, Approaches, and Progress." Mol Plant. 14(1), 61-88.

Lindbo JA. (2007). "TRBO: A High-Efficiency Tobacco Mosaic Virus RNA-Based Overexpression Vector." Plant Physiol. 145(4), 1232-1240.

Zvereva AS, Pooggin MM. (2012). "Silencing and Innate Immunity in Plant Defense Against Viral and Non-Viral Pathogens." Viruses. 4(11), 2578-2597.

Mao Y, et al. (2019). "Application of the CRISPR-Cas System for Efficient Genome Engineering in Plants." Mol Plant. 12(9), 1137-1151.

Cheng X, et al. (2016). "CRISPR/Cas9-Induced Genome Editing and Its Applications in Functional Genomics and Improvement of Crops." Crop J. 4(2), 75-81.

Khabbazi M, et al. (2020). "Virus-Induced Gene Silencing (VIGS) and CRISPR/Cas9-Based Gene Editing in Tomato: Two Powerful Approaches for Functional Genomics Studies." Plants (Basel). 9(1), 50.

Brossard D, et al. (2009). "Science Communication Reconsidered." Nat Biotechnol. 27(6), 514-518.

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Published

2024-02-16

Issue

Section

Review Articles

How to Cite

Harnessing Virus-Induced Gene Silencing for Crop Improvement in Vegetables. (2024). International Journal of Research and Advances in Agricultural Sciences, 3(1), 23-30. https://doi.org/10.8726/nhtge587

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